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Mark Pepper
04-07-03, 10:00 PM
I have gotten a lot of emails lately regarding fruit fly culturing, so I decided I would post this here, seeing as some might find it useful, and because this forum has been slow lately. I will add it to my website in the future.

Culturing fruit flies is relatively easy, and is a cheap alternative to purchasing pinhead crickets.
An easy recipe is as follows
1) Blend together roughly equal parts Banana and Applesauce. When these are blended stir in oatmeal (some cheap variety, plain oatmeal flakes). Mix the oatmeal until the mixture becomes a wet dough-like consistency. (Potato flakes could also be used here)
2) At this time mix in a table spoon of baking yeast. Fish foods can also be added if you like. From here find some old bottles, Snapple Juice bottles, Mason jars etc and put about an inch of the mix in the jar, then sprinkle on top of the mix a few grains of yeast.
3) This next step is important, allow the culture to sit out/open for about 20-24 hours. This allows the yeast to react and release carbon dioxide. Adding the flies before the CO2 has had a chance to dissipate will cause the flies to suffocate.
4) Next add the flies, about 30-40 flies is fine to start a new culture. Then place a layer of cloth with an elastic band over top of the opening, and place the culture in a warm dark place. (75-80F is fine).

In about 5 days you should begin to see many maggots crawling along the sides of the jar. In about another week from the time you have maggots you should have a fresh batch of flies hatching. When these new flies hatch it’s time to start over. (If you are culturing hydei, the whole process takes longer, about one month from the time you start a culture until the time you have flies) It’s best to start new cultures with the freshest flies as they will be most productive. You will need to start about 1 culture for every three frogs you have, every week. This will probably be more than enough but it is better to have too much than too little, these little frogs can eat a lot. If you experience problem with mold, you might consider adding a few drops of diluted methylene blue solution to the mix.

The downside to this recipe is that some say it smells like urine, I prefer to think it smells like a wonderful batch of beer.

This is a recipe I have used for over five or 6 years and have never had any problems with it, or major crashes. I find it produces much better for me than the pre-made mixes that I have tried (I certainly have not tried them all though) and its cheap to make. I got this recipe off of the Frognet mailing list several years ago, it was not a clever idea on my part

Duncan
04-09-03, 05:46 PM
There sure are a lot of fly "recipes" out there aren't there! Mine isn't quite as ambitious as Mark's but I still get a ton of flies and I find the smell stays away for at least 3 weeks (and no mold either). This is the "bulk" recipe (for me anyway) and will make at least 2 dozen cultures.

Mix together: 8 cups of potato flakes
1 cup of icing sugar
1/2 to 1 cup of brewer's yeast

Once its well blended add to a depth of about 3-5cm in the bottom of a plastic beer glass. To this I add (in a 1:1 ratio) a mix of water and apple cider vinegar until a pasty consistency is reached (not too wet or the flies drown, not too dry or the cultures dry out too soon). I add a small piece of nylon window screening for the flies to crawl on, and then cover the beer glass with a coffee filter secured by an elastic band. When the culture starts to "turn," usually no sooner than 3-5 weeks later, I just dump the cup and all into the garbage and start again (no cleaning!). In order to keep a steady supply of flies I actually start about a dozen cultures every 2 weeks. I only have
4 D. tinctorius, 3 E. tricolors, 3 M. expectata, 1 M. pulchra to feed.

At various times I've tried sprinkling yeast on the tops of the cultures, but I found it was too smelly and I don't get any mold with my method anyway (I think the vinegar does the trick). I sometimes wonder about the nutritional value with no fruit in the recipe, but I also feed week old crickets which are well gut loaded.

Anyway, just another method that's out there. I definitely don't have the experience Mark has, but thought I would share a slightly different version of fly culturing.